Main Title |
Plasmid Expression and Maintenance during Long-Term Starvation-Survival of Bacteria in Well Water. |
Author |
Caldwell, B. A. ;
Ye, C. ;
Griffiths, R. P. ;
Moyer, C. L. ;
Morita, R. Y. ;
|
CORP Author |
Oregon State Univ., Corvallis.;Corvallis Environmental Research Lab., OR. |
Publisher |
c1989 |
Year Published |
1989 |
Report Number |
EPA-R-913413; EPA/600/J-89/243; |
Stock Number |
PB90-137050 |
Additional Subjects |
Starvation ;
Aquatic microbiology ;
Water wells ;
Bacteria ;
Enterobacteriaceae ;
Escherichia coli ;
Pseudomonas ;
Graphs(Charts) ;
Reprints ;
Plasmids ;
Gene expression ;
Gene products
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB90-137050 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
7p |
Abstract |
Strains of enteric bacteria and pseudomonads containing plasmid R388::Tn1721(Tpr, Tcr) or pR0101 (Hgr, Tcr) were starved for over 250 days in sterile well water to evaluate effects of starvation-survival on plasmid expression and maintenance. Viable populations dropped to between approximately 0.1 and 1% of the initial populations. Escherichia coli(pR0101) and Pseudomonas cepacia (pR0101) lost both viability and plasmid expression at a lower rate than strains containing R388::Tn1721. Three patterns of host-plasmid interaction were detected: (i) no apparent loss of plasmid expression, (ii) loss of plasmid expression on initial recovery with subsequent expression upon resuscitation, and (iii) loss of capability to produce functional plasmid resistance. (Copyright (c) 1989, American Society for Microbiology.) |