||Effects of Sex and Age on DMBA:DNA Binding in Epidermis of SENCAR Mice Following Topical Administration of Dimethylbenz(a) Anthracene.
Morse, M. A. ;
Baird, W. M. ;
Carlson, G. P. ;
||Purdue Univ., Lafayette, IN. School of Pharmacy and Pharmacal Sciences.;Health Effects Research Lab., Research Triangle Park, NC.;National Institutes of Health, Bethesda, MD.
Deoxyribonucleic acids ;
Skin cancer ;
Skin diseases ;
Age factors ;
Sex factors ;
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Epidermal DMBA+DNA adduct formation was determined in male and female SENCAR mice following short-term exposure to 7,12-dimethylbenz(a)anthracene (DMBA). While no differences in the proportions of the individual adducts were found between male and female mice, male mice generally had higher levels of each individual adduct as well as a greater total binding of DMBA to DNA at 6 to 48 hours after topical administration. Additionally, the effect of age on total DMBA:DNA binding was examined in epidermis of male mice. The levels of binding were greatest between 40 and 54 days of age. These studies demonstrate the importance of considering both the age and sex of animals when examining the metabolism and binding of DMBA and in muscle skin tumor induction experiments using DMBA as an initiator. (Copyright (c) Cancer Letters, 37:1, P187.)