||Fungicide Benomyl(Methyl 1-(Butylcarbamoyl)-2-Benzimidazolecarbamate) Causes Testicular Dysfunction by Inducing the Sloughing of Germ Cells and Occlusion of Efferent Ductules.
Hess, R. A. ;
Moore, B. J. ;
Forrer, J. ;
Linder, R. E. ;
Abuel-Atta, A. A. ;
||Illinois Univ. at Urbana-Champaign. Dept. of Veterinary Biosciences. ;Environmental Protection Agency, Research Triangle Park, NC. Reproductive Toxicology Branch.
Seminiferous tubules ;
Dose-response relationships ;
Reproductive system ;
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Adult male Sprague-Dawley rats (100 days of age) were given single oral doses of the fungicide benomyl (methyl 1-(butylcarbamoyl)-2-benzimidazolecarbamate) in dosages ranging from 25 to 800 mg/kg body weight. The testis and excurrent ducts were examined at 2 and 70 days posttreatment to determine the chemical effects on spermatogenesis and the epididymis. Primary effects seen at 2 days postexposure were testicular swelling and occlusions of the efferent ductules. Mean testis weight peaked with 400 mg/kg. Premature release of germ cells (sloughing), detected even with the lowest dosage (25 mg/kg), was the most sensitive short-term response to benomyl. Sloughing was found primarily in Stage VII of the spermatogenic cycle at the lower dosages, but at higher dosages sloughing extended into all stages except for Stages VIII-XI. Only a few testes were found to be completely regressed. Occlusion of efferent ductules and disruption of the seminiferous epithelium by sloughing of germ cells are overlapping dose-dependent mechanisms responsible for short- and long-term effects of benomyl on the rat testis.