Record Display for the EPA National Library Catalog


Main Title Determination of Giardia cyst viability /
Author Meyer, Ernest A.
CORP Author Oregon Univ. Health Sciences Center, Portland. Dept. of Microbiology and Immunology.;Municipal Environmental Research Lab., Cincinnati, OH.
Publisher Municipal Environmental Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, Available through the National Technical Information Service.
Year Published 1979
Report Number EPA 600-2-79-063; EPA R-804898
Stock Number PB80-108285
OCLC Number 13610144
Subjects Giardia ; Protozoa ; Drinking water--Oregon
Additional Subjects Neoplasms ; Cysts ; Parasites ; Viability ; Determination ; Water ; Protozoa ; Intestines ; Humans ; Animals ; Giardia ; Infections
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EHAM  QD142.M37 Region 1 Library/Boston,MA 04/29/2016
EJBD ARCHIVE EPA 600-2-79-063 Headquarters Library/Washington,DC 04/04/2016
EJBD  EPA 600-2-79-063 Headquarters Library/Washington,DC 01/30/2020
ELBD  EPA 600-2-79-063 AWBERC Library/Cincinnati,OH 06/04/1999
ERAD  EPA 600/2-79-063 Region 9 Library/San Francisco,CA 10/01/2012
ESAD  EPA 600-2-79-063 Region 10 Library/Seattle,WA 07/15/2005
NTIS  PB80-108285 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation viii, 28 pages : illustrations ; 28 cm.
The principal objective of this research was the development of a sensitive, standardized method of determining whether or not Giardia cysts are viable. The availability of such a method is necessary to determine the effect of chemical and physical agents on the viability of these organisms in water. Using cysts collected from an asymptomatic human carrier, a method for inducing excystation was developed and optimized with regard to pH, time of acid exposure, incubation temperature and excystation medium. The method was applied in determining the effects of cyst storage in water at various temperatures on cyst survival as determined by the cultural excystation method and eosin dye exclusion. The results indicate that cyst survival time decreased with increasing temperatures above 0C. Freezing and thawing resulted in almost complete loss of viability.
"EPA 600-2-79-063." "July 1979." Cover title. Prepared for U.S. Environmental Protection Agency, Office of Research and Development, Municipal Environmental Research Laboratory, Cincinnati, Ohio, under grant no. R804898. Includes bibliographical references (pages 24-27).