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Main Title Relationships between Accumulation of a 60 kDa Stress Protein and Scope-for-Growth in 'Mytilus edulis' Exposed to a Range of Copper Concentrations.
Author Sanders, B. M. ; Martin, L. S. ; Nelson, W. G. ; Phelps, D. K. ; Welch, W. ;
CORP Author California State Univ., Long Beach. ;California Univ., San Francisco. Lung Biology Center.;Environmental Research Lab., Narragansett, RI.
Publisher c1991
Year Published 1991
Report Number EPA/600/J-93/315 ; ERLN-1150
Stock Number PB93-229375
Additional Subjects Heat-shock proteins ; Copper ; Mussels ; Growth ; Dose-response relationships ; Western blotting ; Electrophoresis ; Reprints ; Mytilus edulis
Library Call Number Additional Info Location Last
NTIS  PB93-229375 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 19p
Blue mussels, Mytilus edulis, were exposed in the laboratory for 7 days to a range of Cu concentrations (0 to 100 micrograms/liter) to test the feasibility of using the accumulation of a 60kDa stress protein, also referred to as heat shock protein 60 (hsp60), as a molecular indicator of contaminant exposure and ecological status. At the end of the experiment, scope-for-growth (SFG) measurements were completed to assess whole organism physiological condition. In addition, antibodies raised against hsp60 were employed as probes for Western blot analysis and immunobinding assays of mantle tissue. Physiological measurements revealed a significant (p<0.05) decrease in the SFG index for organisms at 32 and 100 micrograms/liter Cu. Western blot analysis indicated that the abundance of hsp60 was greater in tissues exposed to elevated Cu concentrations than in controls. Quantitation of hsp60 accumulation as determined by a dot immunobinding assay demonstrated a significant correlation between hsp60 accumulation and nominal Cu concentration in the seawater. (Copyright (c) 1991 Elsevier Science Publishers Ltd., England.)