||New Standard Guide for Performing the Mouse Lymphoma Assay for Mammalian Cell Mutagenicity.
Clive, D. ;
Caspari, W. ;
Kirby, P. E. ;
Krehl, R. ;
Moore, M. ;
||Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div.
Laboratory animals ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
The purpose and scope of the standard guide is to present background material and to establish criteria by which protocols and procedures for conducting the L5178Y/TK+/- -3.7.2C mouse lymphoma mutagenicity assay (MLA) can be properly understood and evaluated. These guidelines are also intended to aid researchers and others to gain a better understanding of the critical elements involved with mammalian cell mutagenicity testing. More specifically, the document is intended to provide a guide to researchers that will accomplish the following goals: Provide an understanding of the critical procedures (steps) involved in the performance of the mammalian cell mutagenicity test; Provide generalized criteria by which researchers can evaluate if they are properly performing, utilizing, and interpreting the assay; Provide criteria by which individuals responsible for evaluating mouse lymphoma mutagenicity data can determine if the experiments have been properly performed and interpreted.