Record Display for the EPA National Library Catalog

RECORD NUMBER: 19 OF 24

Main Title PCR Primers Specific for Detection of a Rat Repetitive Sequence.
Author Mass, M. J. ; Roop, B. C. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Carcinogenesis and Metabolism Branch.
Publisher c1992
Year Published 1992
Report Number EPA/600/J-92/440;
Stock Number PB93-141398
Additional Subjects Polymerase chain reaction ; Nucleic acid repetitive sequences ; Molecular cloning ; Rats ; Mice ; Cultured cells ; Transfection ; DNA probes ; Reprints ;
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB93-141398 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 4p
Abstract
Identification of a new oncogene is often accomplished by transfection of genomic DNA of interest into a host of a different species that can respond to the altered phenotype. We report a method for tracking and identifying potential genes of interest that are derived from rat DNA. We used polymerase chain reaction to amplify specifically the rat long-interspersed repetitive sequences in mouse cells that were transfected with rat DNA.