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Main Title Identification of Nonpolar Toxicants in Effluents Using Toxicity-Based Fractionation with Gas Chromatography/Mass Spectrometry.
Author Burkhard, L. P. ; Durhan, E. J. ; Lukasewycz, M. T. ;
CORP Author National Effluent Toxicity Assessment Center, Duluth, MN. ;AScI Corp., Duluth, MN.
Publisher c1991
Year Published 1991
Report Number EPA/600/J-91/023;
Stock Number PB91-183434
Additional Subjects Toxicology ; Effluents ; Organic compounds ; Water pollution ; Liquid chromatography ; Mass spectroscopy ; Aquatic animals ; Fractionation ; Lethal dosage ; Validation ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB91-183434 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 8p
A toxicity-based method to identify nonpolar organic toxicants in effluents has been developed. The method has low artifactual toxicity and excellent detection limits, allows multiple toxicant situations to be easily handled, and features the use of cladocerans and fishes as test organisms and gas chromatography/mass spectrometry (GC/MS) to identify the toxicants. The method uses reverse-phase chromatography techniques to extract and fractionate the nonpolar organic toxicants from the effluent. GC/MS analyses are performed on the toxic fractions, and lists of tentative compound identifications are made by interpretation of the mass spectra and elution information from the chromatographic separations. These initial lists are refined by assembling and then comparing toxicity data of the identified chemicals to the toxicity of the fraction. The refined lists of suspect chemicals are further evaluated by pure chemical toxicity testing, and this process ultimately leads to toxicant identification. The fractionation scheme, instrumental parameters, the toxicant identification process, an example illustrating the method, and discussion relevant to the method are presented. (Copyright (c) 1991 American Chemical Society.)