||Activation of the lac Genes of Tn951 by Insertion Sequences from 'Pseudomonas cepacia'.
Wood, M. S. ;
Lory, C. ;
Lessie, T. G. ;
||Massachusetts Univ. at Boston.;Environmental Research Lab., Gulf Breeze, FL.;National Science Foundation, Washington, DC.
||EPA-R-815308 ;NSF-DMB-8415028; EPA/600/J-90/111;
DNA insertion elements ;
Gene expression regulation ;
Nucleic acid sequence homology ;
Messenger RNA ;
Genetic transcription ;
||Some EPA libraries have a fiche copy filed under the call number shown.
Several transposable gene-activating elements from Pseudomonas cepacia have been identified on the basis of their ability to increase expression of the lac genes of the broad-host-range plasmid pGC91.14. When introduced into auxotrophic derivatives of P. cepacia 249 (ATCC 17616), the plasmid failed to confer ability to utilize lactose or lactulose (4-0-beta-galactopyransoyl-D-fructose). The lac genes of the Tn951 element on pGC91.14 were poorly expressed in P. cepacia and were not inducible by IPTG. Lac+ and lactulose+ variants of the pGC91.14-containing transcripients which expressed the lac genes of Tn951 constitutively were isolated as a consequence of transposition of insertion sequences from the P. cepacia genome to sites upstream of the activated genes. The results indicate that IS elements from P. cepacia have potential for turning on the expression of foreign genes in a variety of gram-negative bacteria. (Copyright (c) 1990, American Society for Microbiology.)