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RECORD NUMBER: 16 OF 25

Main Title Mutagenicity of Cyclopenta-Fused Isomers of Benz(a)anthracene in Bacterial and Rodent Cells and Identification of the Major Rat Liver Microsomal Metabolites.
Author Nesnow, S. ; Leavitt, S. ; Easterling, R. ; Watts, R. ; Toney, S. H. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1984
Report Number EPA/600/J-84/260;
Stock Number PB85-193969
Additional Subjects Mutagens ; Bacteria ; Metabolism ; Bioassays ; Aromatic polycyclic hydrocarbons ; Environmental surveys ; Laboratory animals ; Rats ; Liver ; Toxicity ; Public health ; Exhaust emissions ; Chemical analysis ; Molecular structure ; Reprints ; Benzaceanthrylene ; Benzacephenanthrylene
Holdings
Library Call Number Additional Info Location Last
Modified		 Checkout
Status
NTIS  PB85-193969 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 14p
Abstract
The microsomal metabolites and mutagenic activity of four cyclopenta-fused benz(a)anthracenes; benz(j)aceanthrylene (B(j)A), benz(e)aceanthrylene (B(e)A), benz(l)aceanthrylene (B(l)A) and benz(k)acephenanthrylene (B(k)A) have been studied. Arocolor-1254 induced rat liver microsomes metabolized B(j)A to: B(j)A-1,2-dihydrodiol, B(j)A-9,10-dihydrodiol, B(j)A-11,12-dihydrodiol and 10-hydroxy-B(j)A; B(e)A to: B(e)A-1,2-dihydrodiol, B(e)A-3,4-dihydrodiol, and B(e)A-5,6-dihydrodial; B(l)A to: B(l)A-1,2-dihydrodiol, B(l)A-4,5-dihydrodiol and B(l)A-7,8-dihydrodiol; and B(k)A to B(k)A-4,5-dihydrodiol and B(k)A-89-dihydrodiol. With each PAH, metabolism occurred on the cyclopentaring. All four isomers were active as gene mutagens in S. typhimurium and in Chinese hamster V79 cells. In the S. typhimurium mutation studies using Aroclor-1254 induced rat liver S9, B(j)A, B(e)A, and B(l)A required significantly less microsomal protein for maximal mutation response than B(k)A and B(a)P suggesting a one-step activation mechanism, presumably on the cyclopenta-fused ring. B(j)A, B(e)A, and B(l)A were significantly more mutagenic than B(k)A and B(a)P in S. typhimurium.