Record Display for the EPA National Library Catalog


Main Title New Medium for Improved Recovery of Coliform Bacteria from Drinking Water.
Author LeChevallier, M. W. ; Cameron, S. C. ; McFeters, G. A. ;
CORP Author Montana State Univ., Bozeman. Dept. of Microbiology.;Environmental Protection Agency, Cincinnati, OH. Water Engineering Research Lab.
Year Published 1983
Report Number EPA-R-807092; EPA/600/J-83/308;
Stock Number PB85-237410
Additional Subjects Bacteria ; Coliform bacteria ; Culture media ; Potable water ; Cultures(Biology) ; Reprints ; Drinking water
Library Call Number Additional Info Location Last
NTIS  PB85-237410 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 10p
A new membrane filter medium was developed for the improved recovery of injured coliforms from drinking water. The new medium, termed m-T7, consists of 5.0 g of Difco Proteose Peptone no. 3,20 g of lactose, 3.0 g of yeast extract, 0.4 ml of Tergitol 7 (25% solution), 5.0 g of polyoxyethylene ether W-1, 0.1 g of bromthymol blue, 0.1 g of bromcresol purple, and 15 g of agar per liter of distilled water. Additional selectivity may be obtained by aseptically adding 0.1 micrograms of penicillin G per ml to the medium after autoclaving. In laboratory studies, m-T7 agar recovered 86 to 99% more laboratory-injured coliforms than did m-Endo agar. m-T7 agar also recovered an average of 43% more verified coliforms from 67 surface and drinking water samples than did the standard m-Endo membrane filter technique. From drinking water, m-T7 agar recovered nearly three times more coliforms than did m-Endo agar.