Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Quantitative Analysis of the Metabolism of Benzo(a)pyrene by Transformable C3H10T1/2CL8 Mouse Embryo Fibroblasts.
Author Rudo, K. ; Ellis, S. ; Bryant, B. J. ; Lawrence, K. ; Curtis, G. ;
CORP Author Environmental Health Research and Testing, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1986
Report Number EPA/600/J-86/221;
Stock Number PB87-170346
Additional Subjects Pyrenes ; Aromatic polycyclic hydrocarbons ; Metabolism ; Embryos ; Mice ; Laboratory animals ; Cell morphology ; Transformations ; Toxicity ; Carcinogens ; Mutagens ; Quantitative analysis ; Reprints ; Benzopyrenes ; Fibroblasts ; Metabolites
Library Call Number Additional Info Location Last
NTIS  PB87-170346 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/21/1988
Collation 15p
The metabolism of benzo(a)pyrene (B(a)P) to organic-soluble and water-soluble metabolites by transformable C3H10T1/2CL8 mouse embryo fibroblasts was studied as a function of time, B(a)P concentration, and cell density. The total formation of organic-soluble and water-soluble metabolites increased with incubation time from 4 to 48 h and with B(a)P concentration from 4 to 40 micromolarity. As cell density increased, the metabolic rate decreased for organic-soluble and water-soluble products between 6,300 and 54,00 cells/sq cm probably due to decreases in B(a)P concentrations to values below saturation. Specific organic-soluble metabolites identified were B(a)P-pre-9,10-diol, B(a)P-7,8-diol, B(a)P-3,6-quinone, B(a)P-3-phenol, and B(a)P-9-phenol. Water-soluble metabolites were subjected to enzymatic hydrolysis with beta-glucuronidase and aryl sulfatase to identify specific conjugated products. The sulfate conjugated metabolites identified were B(a)P-7,8-diol, B(a)P-pre-9,10-diols, B(a)P-9,10-diol, and B(a)P-3,6-quinone. The beta-glucuronic acid metabolites identified were Ba(a)P-pre-9,10-diols, B(a)P-3,6-quinone, and B(a)P-3-phenol. Patterns of metabolite formation rates are discussed as to their possible effect on morphological transformation rates in C3H10T1/2 cells with respect to incubation time and cell density. (Copyright (c) 1986 Alan R. Liss, Inc.)