Record Display for the EPA National Library Catalog


Main Title Evaluation of m-T7 Agar as a Fecal Coliform Medium
Author LeChevallier, M. W. ; Jakanoski, P. E. ; Camper, A. K. ; McFeters, G. A. ;
CORP Author Montana State Univ., Bozeman. Dept. of Microbiology.;Environmental Protection Agency, Cincinnati, OH. Drinking Water Research Div.
Year Published 1984
Report Number EPA-R-807092; EPA/600/J-84/321;
Stock Number PB85-235166
Additional Subjects Coliform bacteria ; Culture media ; Agar ; Sewage ; Feces ; Acclimatization ; Temperature ; Recovery ; Isolation ; Evaluation ; Reprints ; m-T/7 agar
Library Call Number Additional Info Location Last
NTIS  PB85-235166 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 6p
The m-T/7 agar, designed to improve recoveries of injured total coliforms, was evaluated for its effectiveness as a fecal coliform medium. The time and temperature of preincubation were found to be crucial to the optimal recovery of fetal coliforms. Isolation rates for fecal coliforms on m-T7 agar from sewage effluents were the highest when plates were preincubated at 37C for 8 h before transfer to 44.5C for 12 h. The medium was found to produce consistently higher fecal coliform counts than all the other methods tested. Recoveries were 3.1 times greater than the standard m-FC method and 1.7 times greater than the two-layer enrichment, temperature acclimation procedure. Verification rates for fecal coliforms isolated on m-T7 agar averaged 89.0%, whereas verification rates for m-FC agar averaged only 82.8%. (Copyright (c) 1984, American Society for Microbiology.)