Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Comparison of Extraction Methods for the Isolation of Lipids and PCBs from Mussel Homogenate.
Author Latimer, J. S. ; Quinn., J. G. ;
CORP Author Rhode Island Univ., Narragansett. Graduate School of Oceanography.;Environmental Research Lab., Narragansett, RI.
Publisher Dec 90
Year Published 1990
Report Number EPA/600/3-90/092 ; ERLN-X175
Stock Number PB91-127787
Additional Subjects Mussels ; Lipids ; Graphs(Charts) ; Tables(Data) ; Chemical analysis ; Thin layer chromatography ; Extraction ; Polychlorobiphenyl compounds ; Environmental monitoring ; Bioaccumulation
Library Call Number Additional Info Location Last
NTIS  PB91-127787 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 03/04/1991
Collation 48p
A standard mussel homogenate was analyzed for total lipid mass, lipid class components, and PCBs using four different extraction procedures in an attempt to determine the most appropriate method for use in bioaccumulation studies of lipophilic organic pollutants. The four procedures included: chloroform/methanol/water (similar to the classical Bligh and Dyer method), hexane/acetonitrile/water, and hexane/methanol/water extractions at controlled v/v/v ratios; and a hexane/acetonitrile method requiring minimal solvent volume ratio control. TLC analysis of the lipids, alone, was insufficient to determine qualitative distinctions between the extraction procedures studied; however, Iatroscan(TM) technology was able to provide information on both the qualitative and quantitative distribution of lipids in the various extracts.