Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Comparison of Age-Related Changes in In vivo and In vitro Measures of Testicular Steroidogenesis after Acute Cadmium Exposure in the Sprague-Dawley Rat.
Author Phelps, P. V. ; Laskey, J. W. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1989
Year Published 1989
Report Number EPA/600/J-89/282;
Stock Number PB90-185257
Additional Subjects Cadmium ; Testosterone ; Toxicology ; Aging ; Testis ; Rats ; In vitro analysis ; In vivo analysis ; Animal physiology ; Reprints ; Dose-response relationships ; Acute exposure
Library Call Number Additional Info Location Last
NTIS  PB90-185257 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/15/1990
Collation 14p
Previous reports have demonstrated that cadmium- (Cd-) induced testicular necrosis is an age-dependent process. However, little information exists on age-related intestitial cell (IC) damage in the rat after acute exposure to Cd. In vitro and in vivo measures of testicular damage were utilized to compare the sensitivity of these measures and to further investigate age-related Cd-induced testicular damage. Testes, epididymides, and seminal vesicle weights, serum testosterone (sT), hCG-stimulated sT, and basal and stimulated IC testosterone (T) production were compared in rats 21 d following an injection of 2 mg Cd/kg at 9, 37, 67, and 97 d of age. The only Cd-related change noted for immature rats was an 84% reduction in sT. In rats injected when 37 d old, hCG-stimulated sT and epididymides and seminal vesicle weights, although depressed, were not significantly altered. However, all other measurements were significantly depressed. All measures of testicular damage were significantly depressed in rats injected at 67 and 97 d of age. Overall, in vitro measures were more sensitive indicators of Cd-induced testicular damage than in vivo measures.