Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Efficiency of Beef Extract for the Recovery of Poliovirus from Wastewater Effluents.
Author Landry, Edward F. ; Vaughn, James M. ; Thomas, McHarrell Z. ; Vicale, Thomas J. ;
CORP Author Brookhaven National Lab., Upton, NY.;Health Effects Research Lab., Cincinnati, OH.
Year Published 1978
Report Number EPA-R-804776-01; EPA-600/J-78-183;
Stock Number PB81-213126
Additional Subjects Polioviruses ; Waste water ; Effluents ; Efficiency ; Viruses ; Filters ; Reprints ; Beef extract ; Sewage treatment effluents ; NTISEPAORD
Library Call Number Additional Info Location Last
NTIS  PB81-213126 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/23/1988
Collation 7p
The efficiency of poliovirus elution from fiber glass cartridge filters (K27), epoxy-fiber glass-asbestos filters (M780), and pleated cartridge filters was assessed by using 3% beef extract (pH 9.0) or 0.1 M glycine (pH 11.5). Poliovirus type I, strain LSc, was seeded into 20- to 25-gallon (ca. 75.6- to 95.6-liter) samples of treated sewage effluent and concentrated by using a filter adsorption-elution technique. Virus elution was accomplished by using either two 600-ml portions of 3% beef extract (pH 9.0), or two 1-liter portions of 0.1M glycine (pH 11.5). In all experiments, beef extract elution followed by organic flocculation was found to be superior, yielding a mean recovery efficiency of 85%, with recoveries ranging from 68 to 100%. Elution with 0.1 M glycine (pH 11.5) followed by inorganic flocculation resulted in a mean recovery efficiency of 36%. The variable range of recoveries with beef extract could not be significantly improved by varying the type of beef extract or by extending the elution time to 30 min. Second-step reconcentration of 1-liter seeded sewage effluent and renovated wastewater samples indicated that organic flocculation was a more efficient method for virus recovery than inorganic flocculation. Beef extract concentrations of less than 3% were found to be efficient in the recovery of poliovirus from renovated wastewater.