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Main Title Principles of fluorescence spectroscopy /
Author Lakowicz, Joseph R.
Publisher Springer,
Year Published 2006
OCLC Number 71336464
ISBN 9780387312781; 0387312781
Subjects Fluorescence spectroscopy ; Espectroscopia ; Análise espectroquímica ; Fluoreszenzspektroskopie ; Fluorescentie ; Fluorometrie ; Laser spectrometrie ; Spectrometry, Fluorescence ; Anâalise espectroquâimica
Internet Access
Description Access URL
Contributor biographical information
Publisher description
Library Call Number Additional Info Location Last
EKCM  QD96.F56L34 2006 CEMM/GEMMD Library/Gulf Breeze,FL 01/04/2010
Edition 3rd ed.
Collation xxvi, 954 pages : illustrations ; 29 cm + 1 CD-ROM (4 3/4 in.)
Includes bibliographical references and index.
Contents Notes
Introduction to fluorescence -- Instrumentation for fluorescence spectroscopy -- Fluorophores -- Time-domain lifetime measurements -- Frequency-domain lifetime measurements -- Solvent and environmental effects -- Dynamics of solvent and spectral relaxation -- Quenching of fluorescence -- Mechanisms and dynamics of fluorescence quenching -- Fluorescence anisotropy -- Time-dependent anisotropy decays -- Advanced anisotropy concepts -- Energy transfer -- Time-resolved energy transfer and conformational distributions of biopolymers -- Energy transfer to multiple acceptors in one, two, or three dimensions -- Protein fluorescence -- Time-resolved protein fluorescence -- Multiphoton excitation and microscopy -- Fluorescence sensing -- Novel fluorophores -- DNA technology -- Fluorescence-lifetime imaging microscopy -- Single-molecule detection -- Fluorescence correlation spectroscopy -- Radiative decay engineering : metal-enhanced fluorescence -- Radiative decay engineering : surface plasmon-coupled emission -- Appendix I. Corrected emission spectra -- Appendix II. Fluorescent lifetime standards -- Appendix III. Additional reading -- Answers to problems. Organized as a textbook for the learning student or the researcher needing to acquire the core competencies, this volume maintains the emphasis on basics, while updating the examples to include recent results from the literature. The third edition also includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. This full-color textbook features the following: problem sets following every chapter; glossaries of commonly used acronyms and mathematical symbols; appendices containing a list of recommended books which expand on various specialized topics; and, sections describing advanced topics will indicate as such, to allow these sections to be skipped in an introductory course, allowing the text to be used for classes of different levels.