Record Display for the EPA National Library CatalogRECORD NUMBER: 2 OF 3
|Main Title||Determination of the sites(s) [i.e. site(s)] of action of selected pesticides by an enzymatic-immunobiological approach /|
|Author||Koch, Robert B.|
|CORP Author||Mississippi State Univ., Mississippi State. Dept. of Biochemistry.;Environmental Research Lab., Gulf Breeze, FL.|
|Publisher||Environmental Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency ; National Technical Information Service [distributor],|
|Report Number||EPA-600/3-78-093; EPA-R-803458|
|Stock Number||PB-289 969|
|Subjects||Immunoenzyme technique. ; Pesticides--Toxicology.|
|Additional Subjects||Pesticides ; Antibodies ; Development ; Chlorine organic compounds ; Inhibition ; Recommendations ; Rabbits ; Chickens ; Laboratory animals ; Antigen antibody reactions ; Enzymes ; Insecticides ; Chlorine organic compounds ; Kelevan ; Adenosine triphosphatase ; Kepone ; Immune serums ; Methenocyclobutapentalene levulinic acid/decachlorooctahydro-hydroxy-(ethyl-ester) ; Cyclobutapentalenone/decachlorocathydro|
|Collation||vii, 29 pages : illustrations ; 28 cm.|
The report describes development of an antibody to an organochlorine pesticide to be used in studies related to its inhibition of the ATPase system. Kelevan, the condensation product of ethyl levulinate and Kepone, was successfully conjugated to bovine serum albumin (BSA), fibrinogen (BF), and gamma globulin (BGG). Rabbits and chickens preimmunized with BSA and then immunized with BSA-Kelevan produced antibodies to both the hapten, Kelevan, and the carrier protein BSA. Antiserum to Kelevan protected ATPase activity against Kepone and its derivatives. The titer of antibody to Kelevan was critical since antiserum with only trace amounts of Kelevan antibody failed to protect the ATPase activity against Kepone Inhibition. Antibody was concentrated by Na2S04 fractional precipitation of the antiserum and obtained in pure form by affinity chromatography using BGG-Kel covalently linked to Sepharose 4B. Pure antibody was obtained from untreated blood serum or plasma with no prior pretreatment or fractionation using the BGG-Kel affinity column. Complete protection of mitochondrial mg2+ATPase activity from in vitro inhibition of Kepone was obtained using a 1.2 mg quantity of na2S04 fractionated antibody and only 120 micrograms of pure antibody. Reversal of ATPase inhibition was readily obtained by addition of antibody prior to addition of substrate to the reaction mixture.
"Mississippi State University, Department of Biochemistry." "Environmental Research Laboratory, U.S. Environmental Protection Agency, Gulf Breeze, Florida." "October 1978." Includes bibliographical references (pages 27-29). "Grant no. R803458." Microfiche.