| Main Title |
Co-Culture of Rat Embryos and Hepatocytes: 'In vitro' Detection of a Proteratogen. |
| Author |
Oglesby, L. A. ;
Ebron, M. T. ;
Carver, B. D. ;
Kavlock, R. J. ;
Beyer, P. E. ;
|
| CORP Author |
Northrop Services, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. |
| Year Published |
1986 |
| Report Number |
EPA-68-02-4032; EPA/600/J-86/070; |
| Stock Number |
PB86-213766 |
| Additional Subjects |
Toxicology ;
In vitro analysis ;
Rats ;
Detection ;
Embryos ;
Hepatocytes ;
Proteratogens
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB86-213766 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
12p |
| Abstract |
Rat embryos removed from the dam on day 10 of pregnancy were successfully co-cultivated in vitro with primary cultures of rat, rabbit, or hamster hepatocytes. Embryos co-cultivated with hepatocytes developed normally, as did embryos exposed to a test chemical, cyclophosphamide. In a system where embryos were co-cultivated with hepatocytes and then exposed to cyclophosphamide a dose-related embryotoxicity was observed, indicating metabolic activation of the proteratogen. When similar numbers of hepatocytes were used for metabolic activation of cyclophosphamide, the induced embryo-toxicity was greater in the presence of rabbit and hamster hepatocytes than with rat hepatocytes. |
