||Rodent Whole-Embryo Culture as a Teratogen Screening Method (Journal Version).
Kitchin, K. T. ;
Schmid, B. P. ;
Sanyal, M. K. ;
||Health Effects Research Lab., Research Triangle Park, NC. ;Yale Univ., New Haven, CT. School of Medicine.
Drug screening ;
Laboratory animals ;
Tissue culture ;
Fetal development ;
Postimplantation phase ;
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In vitro rodent whole embryo culture offers a wholistic system in which extremely rapid cell division and specific time-related morphogenic events occur. Because in vitro development so closely parallels that occurring in vivo, the in vitro embryo culture system appears to be particularly relevant in the detection of teratogenic and embryotoxic compounds. Correlation between the in vitro screen and the published results of in vivo embryo toxicology and teratology studies has been very good. There is a high correlation (r=0.97) between the concentration of teratogenic agents which cause adverse effects in vitro and the in vivo teratogenic dose of these compounds.