Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Characterization of an Axenic Strain of 'Hartmannella vermiformis' Obtained from an Investigation of Nosocomial Legionellosis.
Author Fields, B. S. ; Nerad, T. A. ; Sawyer, T. K. ; King, C. H. ; Barbaree, J. M. ;
CORP Author Center for Infectious Diseases, Atlanta, GA. Div. of Bacterial Diseases. ;American Type Culture Collection, Rockville, MD. ;Rescon Associates, Inc., Royal Oak, MD. ;Georgia Univ., Athens. Coll. of Veterinary Medicine.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Publisher c1990
Year Published 1990
Report Number EPA-R-816070-01-0; EPA/600/J-90/525;
Stock Number PB91-211425
Additional Subjects Hartmanella ; Cross infections ; Legionellosis ; Legionella ; Isoenzymes ; Water microbiology ; Polyacrylamide gel electrophoresis ; Reprints ; Protozoan-bacterial interactions
Library Call Number Additional Info Location Last
NTIS  PB91-211425 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 11/26/1991
Collation 5p
A free living amoeba identified as Hartmannella vermiformis was isolated from a water sample obtained during an investigation of nosocomial legionellosis. Hartmannella vermiformis are known to support the intracellular multiplication of Legionella pneumophila. This strain of H. vermiformis, designed CDC-19, was cloned and established in axenic culture, in order to develop a model for the study of the pathogenicity of legionellae. Isoenzyme patterns of axenically cultivated strain CDC-19 were compared with 2 strains of H. vermiformis derived from the type strain, one axenic (ATCC 50236) and the other grown in the presence of bacteria (ATCC 30966). Enzyme patterns suggested that all 3 strains are assignable to the species Hartmannella vermiformis. Axenic H. vermiformis strain CDC-19 has been deposited with the American Type Culture Collection (ATCC 50237) and should prove useful in the study of protozoan-bacterial interaction. (Copyright (c) 1990 American Society of Protozoologists.)