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Main Title The detection of radicals produced 'in vivo' during inhalation exposure to ozone: use of various spin traps. /
Author Kennedy, C. H. ; Hatch, G. E. ; Slade, R. ; Mason, R. P.
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;National Inst. of Environmental Health Sciences, Research Triangle Park, NC.
Publisher US Environmental Protection Agency, Office of Research and Development, Health Effects Research Laboratory,
Year Published 1991
Report Number EPA/600/D-91/111
Stock Number PB91-196113
Additional Subjects Free radicals ; Ozone ; Air pollution effects(Animals) ; Rats ; Electron spin resonance ; Pulmonary edema ; Carbon dioxide ; Lipid peroxidation
Library Call Number Additional Info Location Last
NTIS  PB91-196113 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9 pages ; 29 cm
Ozone is known to induce lipid peroxidation of lung tissue, although no direct evidence of free radical formation has been reported. The study used the electron paramagnetic resonance (EPR) spin-trapping technique to search for free radicals produced in vivo by ozone exposure. The spin trap alpha-(4-pyridyl 1-oxide)-N-tert-butylnitrone (4-POBN) was administered i.p. to male Sprague-Dawley rats. The rats were then exposed for 2 hrs to either 0, 0.5, 1.0, 1.5 or 2.0 ppm ozone with 8% CO2 to increase their respiratory rate. A 6-line 4-POBN/radical spin adduct signal a(sup N)=15.02 G and a(sup H)beta-3.27 G) was detected by EPR spectroscopy in lipid extracts from lungs of rats treated with 4-POBN and then exposed to ozone. Only a very weak signal was observed in the corresponding solution from rats exposed to 0 ppm ozone (air with CO2 only). The concentration of the radical adduct increased as a function of ozone concentration. After administration of 4-POBN, rats were exposed for either 0.5, 1.0, 2.0 or 4.0 hrs to either 0 or 2.0 ppm ozone (with CO2). These results demonstrate that ozone induces the production of free radicals in rat lungs during inhalation exposure and that radical production may be involved in the induction of lung edema by ozone.
"EPA 600/D-91/111." Microfiche.