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Main Title Pharmacologic Probing of Amphotericin B-Induced Renal Dysfunction in the Neonatal Rat.
Author Gray, J. A. ; Kavlock, R. J. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Perinatal Toxicology Branch. ;North Carolina State Univ. at Raleigh.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/486;
Stock Number PB90-185208
Additional Subjects Pharmacology ; Toxicology ; Diuretics ; Rats ; Graphs(Charts) ; pH ; Diuresis ; Reprints ; Amphotericin B ; Kidney diseases ; Newborn animals ; Physiopathology
Library Call Number Additional Info Location Last
NTIS  PB90-185208 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
Acetazolamide, furosemide, chlorothiazide, and amiloride are pharmacologic agents that act primarily in the proximal tubule, loop of Henle, early distal tubule and late distal tubule and collecting duct, respectively. In order to investigate the renal pathophysiology induced by amphotericin B, these diuretic agents were used as probes of discrete segments of the nephron in the neonatal rat. Six-day-old rats were treated with amphotericin B (20 mg/kg, sc) or the vehicle. Twenty-four hours later, when evidence of amphotericin B-induced renal pathophysiology is detectable, the responses to the diuretic agents were assessed in a 2-hr clearance test, during which creatinine clearance (CCr) and the fractional excretion (FE) of water and various components of the filtrate were determined. Amphotericin B induced alterations in basal function including azotemia, hypostenuria, increases FE water and electrolytes, and a decreased FE urea (although CCr was normal). All of the diuretic agents elicited an increase in urea excretion in amphotericin B-treated pups such that FE urea approached control values. (Copyright (c) 1988 Academic Press, Inc.)