Record Display for the EPA National Library Catalog


Main Title Turnover of Extracellular DNA in Eutrophic and Oligotrophic Freshwater Environments of Southwest Florida.
Author Paul, J. H. ; Jeffrey, W. H. ; David, A. W. ; DeFlaun, M. F. ; Cazares., L. H. ;
CORP Author University of South Florida, St. Petersburg. Dept. of Marine Science.;Environmental Research Lab., Gulf Breeze, FL.;National Science Foundation, Washington, DC.
Publisher c1989
Year Published 1989
Report Number NSF-BSR86-01570; EPA/600/J-89/175;
Stock Number PB90-140823
Additional Subjects Deoxyribonucleic acids ; Fresh water ; Genetics ; Water microbiology ; Plankton ; Florida ; Biomass ; Tables(Data) ; Graphs(Charts) ; Aquatic microbiology ; Reprints ; Extracellular matrix ; Genetic engineering ; Chlorophyll ; Base sequence
Library Call Number Additional Info Location Last
NTIS  PB90-140823 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 8p
Turnover of extracellular DNA was investigated in oligotrophic springs of the Crystal River and the eutrophic Medard Reservoir of southwest Florida. The Medard Reservoir possessed large populations of bacterioplankton and phytoplankton (6.8 x 10 sup 9 cells per liter and 28.6 micrograms of chlorophyll a per liter, respectively), while the Crystal River springs only contained a fraction of the microbial biomass found in the Medard Reservoir. The results indicate that regardless of trophic status or microbial standing stock, extracellular DNA turns over rapidly in subtropical planktonic freshwater environments. Therefore, recombinant DNA sequences from released genetically engineered microorganisms might not be expected to survive for long periods of time in freshwater planktonic environments. (Copyright (c) 1989 American Society for Microbiology.)