The document presents test procedures for the assessment of the biological efficacy of pesticides against several groups of important insect pests which pose an economic and public health threat to man in urban, rural, agricultural and wilderness habitats. While these protocols do not cover all procedures and techniques employed to date, they, however, include the most widely used and accepted procedures by researchers in this field. Deviation from and modification of these procedures may be deemed desirable when required by specific conditions. The insects for which evaluation procedures are outlined, belong to four families: Culicidae, Simuliidae, Ceratopogonidae, and Chironomidae. The procedures and techniques outlined are mere guidelines for securing meaningful information on the efficacy of pesticides for the control of pest and vector insects.