Record Display for the EPA National Library Catalog


Main Title Recovery of 'Giardia' Cysts from Water: Centrifugation Versus Filtration.
Author Holman, B. ; Frost, F. ; Plan, B. ; Fukutaki, K. ; Jakubowski, W. ;
CORP Author Washington State Dept. of Social and Health Services, Olympia.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1983
Report Number EPA-R-805809; EPA-600/J-83-152;
Stock Number PB84-153717
Additional Subjects Cysts ; Centrifuging ; Filtration ; Protozoa ; Reprints ; Giardia lamblia ; Pathogens ; Water pollution detection ; Monitoring
Library Call Number Additional Info Location Last
NTIS  PB84-153717 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 6p
Methods for the recovery of Giardia cysts from large volume (1900 L) water samples were experimentally examined in the laboratory and applied to 22 surface water supplies around Washington state. Orlon yarn-wound filters (7 and 1 micrometer porosity) were compared, with higher cyst recovery rates observed for 1 micrometer filters. For each experiment, half the recovered material was processed through a series of screens while the other half was processed through an algae (Foerst) centrifuge. Both methods recovered cysts from 1900 L water samples containing as few as 6000 cysts, with higher recovery rates observed for the algae centrifuge. Cysts were recovered more often from surface water sources implicated in outbreaks (3 of 4) than from sources not implicated in known outbreaks (2 of 18).