||Separation and Detection of a Benzo(a)pyrene Deoxyguanosy-5-monophosphate Adduct by Capillary Zone Electrophoresis.
Jackim, E. ;
Norwood, C. ;
||Environmental Research Lab., Narragansett, RI.
Thin layer chromatography ;
Ultraviolet spectroscopy ;
DNA adducts ;
High pressure liquid chromatography ;
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The detection of DNA adducts from environmentally exposed organisms is difficult because of their low concentration (one adduct in 10 to the 7th power - 10 to the 9th power normal nucleotides). The most feasible way of detecting these adducts has been by radioactive P(sup 32) post labeling and separation by multidimensional thin layer chromatography, or more recently by monitoring the radioactivity after HPLC separation. The procedure lacks precision, is difficult to perform, and poses health risks because of the high specific P(sup 32) radioactivity required. Capillary Zone Electrophoresis (CZE) with fluorescent or indirect fluorescent detection offers the potential of comparable detection sensitivity with much greater resolution if the adducts can be separated. Here evidence is present that a benzo(a)pyrene guanosine monophosphate adduct can be separated from normal nucleotides and detected by CZE using a UV absorption detector. (Copyright (c) Dr. Alfred Huethig Publishers.)