Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Induction of Mutations by Chemical Agents at the Hypoxanthine-Guanine Phosphoribosyl Transferase Locus in Human Epithelial Teratoma Cells.
Author Huberman, E. ; McKeown, C. K. ; Jones, C. A. ; Hoffman, D. R. ; Murao, S. ;
CORP Author Argonne National Lab., IL. ;Oak Ridge National Lab., TN.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1984
Report Number EPA/600/J-84/357;
Stock Number PB86-162237
Additional Subjects Toxicology ; Humans ; Cells(Biology) ; Chromosomes ; Reprints ; Mutagens ; Thioguanine ; Cell lines
Library Call Number Additional Info Location Last
NTIS  PB86-162237 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/21/1988
Collation 13p
Induction of 6-thioguanine (TG) resistance by chemical mutagens was examined in a line of cells derived from a human epithelial teratocarcinoma cell clone. The cells, designated as P3 cells, have a stable diploid karyotype with 46(XX) chromosomes, including a translocation between chromosomes 15 and 20. Efficient recovery of TG-resistant mutants induced by the direct-acting mutagens: N-methyl-N'-nitro-N-nitrosoguanidine (MNNG); 7beta,8alpha-dihydroxy-9alpha,10alpha-epoxy-7,8,9,10-tetrahydrobenzo(alpha)pyrene (BPDE); and benzo(alpha)pyrene (B(alpha)P); activated in a cell-mediated assay, required an expression time of 7 days and a saturation density of 2X10 to the fourth power cells/60-mm petri dish. The TG-resistant mutant cells induced by MNNG and BPDE maintained their resistant phenotype 4-6 weeks after isolation. This mutant phenotype was associated with a morre than 10-fold reduction in hypoxanthine-guanine phosphoribosyl transferase (HGPRT) activity relative to that of the parental P3 cell line, which was shown to catalyze the formation of 4.6 pmoles inosine-5'-monophosphate (IMP)/min/micrograms protein. Induction of TG resistance was also observed in P3 cells cocultivated in a cell-mediated assay with human breast carcinoma cells. Which are capable of polycyclic aromatic hydrocarbon (PAH) metabolism.