Record Display for the EPA National Library Catalog


Main Title Survival, Blood Osmolality, and Gill Morphology of Juvenile Yellow Perch, Rock Bass, Black Crappie, and Largemouth Bass Exposed to Acidified Soft Water.
Author McCormick, J. H. ; Jensen, K. M. ; Leino, R. L. ;
CORP Author Environmental Research Lab.-Duluth, MN. ;AScI Corp., Duluth, MN. ;Minnesota Univ., Minneapolis. Medical School.
Publisher c1989
Year Published 1989
Report Number EPA/600/J-89/317;
Stock Number PB90-198706
Additional Subjects Acidification ; Fishes ; Toxicity ; pH ; Exposure ; Fresh water biology ; Pathology ; Blood analysis ; Histology ; Morphology ; Reprints ; Environmental effects ; Aquatic ecosystems ; Water pollution effects(Animals) ; Biological indicators
Library Call Number Additional Info Location Last
NTIS  PB90-198706 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 15p
When exposed to a range of pH from 7.0 to 4.0 in soft water (1 mg Ca(2+)/L), juvenile rock bass Ambloplites rupestris, black crappie Pomoxis nigromaculatus, and largemouth bass Micropterus salmoides showed a capacity to osmoregulate and survive for up to 30 d at pH 4.5 and above. Juvenile yellow perch Perca flavescens maintained osmoregulatory control through 58 d at pH 5.0. All four species lost osmoregulatory control at pH 4.0, and death of fish ensued within a few days after blood osmolality declined to about 200 mosmol/kg or less (normal values, about 300 mosmol/kg). After 58 d of exposure of pH 4.0, mean blood osmolality of yellow perch was 218 mosmol/kg, and these fish were severely emaciated and moribund. Rock bass, black crappie, and largemouth bass all died by days 29, 16, and 9, respectively, when exposed to pH 4.0. Examination of gills showed progressively increased pathology with longer exposures to lower than normal pH. Among fish exposed to low pH, gill hyperplasia was present most often, but epithelial hypertrophy, chloride-cell proliferation, chloride-cell degeneration, edema, and vacuolization of the tissues also were observed. Morphological changes that were observed in the three centrarchids at pH values above pH 4.0 suggested that gill pathology may be a more sensitive indicator of potentially lethal acid stress than blood osmolality.