Main Title |
Flow Cytometric Analysis of Effects of 1,3-Dinitrobenzene on Rat Spermatogenesis. |
Author |
Evenson, D. P. ;
Janca, F. C. ;
Jost, L. K. ;
Baer, R. K. ;
Karabinus, D. S. ;
|
CORP Author |
South Dakota State Univ., Brookings. Dept. of Chemistry.;Health Effects Research Lab., Research Triangle Park, NC. |
Publisher |
c1989 |
Year Published |
1989 |
Report Number |
EPA-R-810991; EPA/600/J-89/372; |
Stock Number |
PB90-216136 |
Additional Subjects |
Rats ;
Testis ;
Deoxyribonucleic acids ;
Ribonucleic acids ;
Mutations ;
Reprints ;
Flow cytometry ;
Spermatogenesis ;
Dinitrobenzenes ;
Cell nucleus ;
Dose-response relationships ;
Vas deferens ;
Epididymis
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB90-216136 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
20p |
Abstract |
Exposure of 100-d old rats to 1.3-dinitrobenzene (m-DNB) at dosages up to 48 mg/kg resulted in disruption of spermatogenesis as measured by flow cytometry (FCM) of acridine orange-stained sperm and testis cells. One day (d 1) after a single exposure to 48 mg/kg m-DNB. FCM measurements of caput epididymal fluid cells demonstrated the presence of testicular germinal epithelial cells apparently sloughed off into the epididymis. Also, at d 1 after the same exposure, a decrease in pachytene spermatocytes was observed. By d 16 after exposure to 32 or 48 mg/kg, testicular damage was evidenced by an alteration of cell type ratios in FCM-analyzed populations of testicular cells. Extensive recovery of cell type ratios occurred by d 32. At d 16, dosages of 32 and 48 mg/kg caused alterations of sperm chromatin structure as determined by the flow cytometric sperm chromatin structure assay (SCSA); 48 mg/kg caused alterations at both d 16 and d 32. Exposure to m-DNB caused a dose response increase in percent sperm head morphology abnormalities (%ABN) assessed in cauda epididymal and vas sperm. (Copyright (c) 1989 by Hemisphere Publishing Corporation.) |