| Main Title |
Improved Method for the Determination of Aryl-4-hydroxylase Activity. |
| Author |
Lindblad, William J. ;
Jackim, Eugene ;
|
| CORP Author |
Environmental Research Lab., Narragansett, RI. |
| Year Published |
1981 |
| Report Number |
EPA-600/J-81-525 ;ERLN-J185; |
| Stock Number |
PB82-167230 |
| Additional Subjects |
Filtration ;
Separation ;
Enzymes ;
Revisions ;
Toxicology ;
Extraction ;
Fluorescence ;
Cellulose acetate ;
Reprints ;
Hydroxylases/aryl ;
Tritium release assay ;
Benzopyrenes
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB82-167230 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
3p |
| Abstract |
An improved method for separation of tritiated benzo(a) pyrene from tritiated water in the aryl-4-hydroxylase assay is presented. Quantitative retention of benzo(a) pyrene was obtained with mixed cellulose acetate and nitrate filters. Enzymatic activities obtained with this modification agreed well with those determined using the standard charcoal column extraction. This modification greatly reduces the time required to perform this assay, and allows for the determination of enzyme activity in a large number of samples. |
