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Main Title Analysis of Arachidonic Acid Metabolites and Platelet Activating Factor Production.
Author Madden, M. ; Samet, J. ; Koren, H. ; Friedman., M. ;
CORP Author North Carolina Univ. at Chapel Hill.;Environmental Protection Agency, Research Triangle Park, NC.
Publisher Jun 91
Year Published 1991
Report Number EPA-R-812738; EPA/600/D-91/190;
Stock Number PB91-231084
Additional Subjects Arachidonic acids ; Eicosanoids ; Platelet activating factor ; Biochemistry ; Biosynthesis ; High pressure liquid chromatography ; Thin layer chromatography ; Radioimmunoassay ; Pollutants ; Enzyme-linked immunosorbent assay ; Chemical analysis ; Tissue culture ; Cultured cells ;
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NTIS  PB91-231084 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 36p
Abstract
Metabolites of arachidonic acid (eicosanoids) and platelet activating factor are important bioactive lipids that may be involved in the pathobiological alterations in animals induced by pollutant exposure. Analysis of these substances in biological tissue and fluids is important for determining their role in these processes. The manuscript describes and reviews some of the analytical techniques available for quantitation of these lipids. The described assays include use of high performance lipid chromatography, thin layer chromatography, radioimmunoassay, and enzyme-linked immunoassay.