Record Display for the EPA National Library Catalog

RECORD NUMBER: 5 OF 8

Main Title Metabolism of 1-Nitropyrene by Cultured Rabbit Alveolar Macrophages and Respiratory Tract Tissues.
Author King, L. C. ; Ball, L. M. ; Jackson, M. ; Inmon, J. P. ; Lewtas, J. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Northrop Services, Inc., Research Triangle Park, NC.
Year Published 1986
Report Number EPA/600/J-86/032;
Stock Number PB86-190121
Additional Subjects Toxicology ; Metabolism ; Macrophages ; Tissues(Biology) ; Reprints ; Nitropyrene ; Metabolites
Holdings
Library Call Number Additional Info Location Last
Modified		 Checkout
Status
NTIS  PB86-190121 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
Abstract
The metabolism of 1-nitropyrene (1-NP) was studied in cultured rabbit alveolar macrophages and respiratory tissues from both lung and trachea. Metabolites from the incubation medium and from the macrophages and respiratory tissues were isolated and quantitated by HPLC. The following metabolites were isolated and quantitated: 1-nitro-pyrene-4,5- or -9,10-dihydrodiol (K-DHD), N-acetyl-1-aminopyrene (NAAP), 1-aminopyrene (1-AMP), 10-hydroxy-1-nitropyrene (10-OH-1-NP), 4-,5-,6-,8- or 9-monohydroxy-1-nitropyrene (phenols), and 3-hydroxy-1-nitropyrene (3-OH-1-NP). The total percentage of 1-NP metabolism was significantly greater in the lung and tracheal tissue (28.0 to 23.0% of the recovered 14C, respectively) compared to the alveolar macrophages (6.3%). The tracheal tissue was found to have the highest activity both in 1-NP metabolism and intracellular metabolite concentration. A major portion of the 1-NP metabolites produced were released into the incubation medium.