Record Display for the EPA National Library Catalog


Main Title Preparation of Biological Tissue Sections for Correlative Ion, Electron, and Light Microscopy.
Author Kupke, K. G. ; Pickett, J. P. ; Ingram, P. ; Griffis, D. P. ; Linton, R. W. ;
CORP Author Duke Univ. Medical Center, Durham, NC. ;Veterans Administration Medical Center, Durham, NC. ;Research Triangle Inst., Research Triangle Park, NC. ;North Carolina Univ. at Chapel Hill. Dept. of Chemistry.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1984
Report Number EPA-R-807560; EPA/600/J-84/354;
Stock Number PB86-136223
Additional Subjects Electron microscopy ; Microscopy ; Tissues(Biology) ; Reprints ; Ion microscopy
Library Call Number Additional Info Location Last
NTIS  PB86-136223 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 12p
In order to correctly interpret the chemical images obtained using ion microscopy (IM), it is useful to correlate them with the information provided by conventional light microscopy (LM), secondary electron imaging (SEI), backscattered electron imaging (BEI), and electron probe microanalysis (EPMA). Accordingly, the authors have devised a technique of specimen preparation which allows for the application of several different microanalytical techniques to a single histologic section mounted on the same substrate. Sections are cut onto polyester plastic coverslips (devoid of peaks for any element with atomic number >9 using EPMA) and studied by LM. After a light rotary coating with carbon (to prevent charging), the section can then be examined by SEI, BEI, and EPMA. Specific areas can be marked for IM study either with an objective-mounted pin tissue microlocater, or by placing small pieces of metal foil, cut in specific geometric shapes, over features of interest.