Record Display for the EPA National Library Catalog


Main Title Vaporization Technique to Measure Mutagenic Activity of Volatile Organic Chemicals in the Ames/'Salomella' Assay.
Author Hughes, T. J. ; Simmons, D. M. ; Monteith, L. G. ; Claxton, L. D. ;
CORP Author Research Triangle Inst., Research Triangle Park, NC. Center for Life Sciences and Toxicology.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1987
Report Number EPA-68-02-3170 ;EPA-68-02-3992; EPA/600/J-87/163;
Stock Number PB88-154430
Additional Subjects Vaporization ; Toxicology ; Bacteria ; Reprints ; Mutagenesis ; Volatile organic compounds ; Ames Salmonella assay ; Analytical methods
Library Call Number Additional Info Location Last
NTIS  PB88-154430 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 23p
The purpose of the research was to develop and characterize a sensitive test method to detect mutagenic activity of volatile liquid organic chemicals (i.e., volatiles) in the Ames/Salmonella assay. A Tedlar bag vaporization technique was developed which increased contact time between the volatiles and bacterial test system, circumvented volatilization limitations in the standard plate incorporation and preincubation methods, allowed chemical analysis during incubation and was flexible in design. The vaporization technique was evaluated concurrently against the plate incorporation and preincubation techniques with eight liquid volatile mutagens. When volatile organic chemicals with boiling points below 63 C were tested for mutagenic activity, the most sensitive test conditions were the vaporization technique with TA100. GC analysis of epichlorohydrin and butylene oxide concentrations within Tedlar bags suggested that these two chemicals volatilized and were contained in the media after one hour of incubation at 37 C.