Record Display for the EPA National Library Catalog


Main Title Humoral Antibody Response to Individual Viral Proteins After Murine Cytomegalovirus Infection.
Author Selgrade, M. K. ; Huang, Y. S. ; Graham, J. A. ; Huang, C. H. ; Hu, P. C. ;
CORP Author North Carolina Univ. at Chapel Hill.;Health Effects Research Lab., Research Triangle Park.
Year Published 1983
Report Number EPA-600-83-180;
Stock Number PB84-174374
Additional Subjects Viral diseases ; Proteins ; Antibodies ; Reprints ; Cytomegaloviruses ; Immune response
Library Call Number Additional Info Location Last
NTIS  PB84-174374 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 6p
The purpose of this study was to identify viral proteins that played an important role in the humoral immune response to murine cytomegalovirus (MCMV). Viral proteins were separated from a purified virus preparation on polyacrylamide gels, were blotted onto nitrocellulose strips, and were reacted with antisera collected from mice on various days post infection. No antibody response was detected in serum obtained 5 days post infection, but by 10 days there was a faint response to five different proteins. Thereafter, the number of proteins eliciting an antibody response, as well as the intensity of the response, increased with time so that by 42 days post infection a response to 13 major antigens was detected. This method provides a means of separating out important immunogens from the more than 30 different MCMV proteins originally identified by polyacrylamide gel electrophoresis. Such information may improve our understanding of the pathogenesis of MCMV infection as well as host immune responses to the virus.