Record Display for the EPA National Library Catalog


Main Title Experimental Infection of the Respiratory Tract with Mycoplasma Pneumoniae.
Author Hu, Ping-Chuan ; Kirtz, Jerome M. ; Gardner, Donald E. ; Powell, Dwight A. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1981
Report Number EPA/600/J-81-028;
Stock Number PB81-233801
Additional Subjects Respiratory system ; Infectious diseases ; Immunity ; Aerosols ; Hamsters ; Laboratory animals ; Labeled substances ; Lung ; Reprints ; Mycoplasma pneumoniae ; Pathogens
Library Call Number Additional Info Location Last
NTIS  PB81-233801 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 8p
Mycoplasma pneumoniae, a common human respiratory pathogen, has been studied experimentally for years using intranasal inoculation of the golden Sytrian hamster. Because of recent evidence outlining the role in pulmonary immune development of particle size and depth of mycoplasma deposition in the hamster lung, the authors developed an aerosol chamber for the reproducible aerosolization of radiolabeled M. pneumoniae. Organisms were labeled to high specific activity by the incorporation of 3H-oleic acid and aerosolized under air-flow and humidity conditions creating a mean particle diameter of 2.0 micrometers. Under these conditions, viable mycoplasmas were reproducibly and evenly distributed to all major lobes of the lung. Examination of radioactive clearance and organism viability within the lung during the first 48 hr after aerosolization have suggested a minimal role for macrophage mycoplasmacidal activity and a more prominent role for ciliary clearance. Data from aerosol infections of hamsters with radio-labeled M. pneumoniae should provide a unique opportunity to examine in a highly controlled manner the effects of air pollutants on the initial stages of infection as well as effects on the development of pulmonary immunity and histologic alterations.