Record Display for the EPA National Library Catalog


Main Title High Resolution Cytogenetic Characterization of the L5178Y TK+/- Mouse Lymphoma Cell Line.
Author Sawyer, J. R. ; Hoyle, K. M. ; Moore, M. M. ; Hozier, J. C. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div.;Florida Inst. of Tech., Melbourne. Medical Genetics Lab.
Publisher c1989
Year Published 1989
Report Number EPA/600/J-89/365;
Stock Number PB90-215948
Additional Subjects Chromosomes ; Mutations ; Mice ; Lymphomas ; Reprints ; Chromosome aberrations ; Thymidine kinase ; Cultured tumor cells ; Acridine orange ; Chromosome banding ; Karyotyping
Library Call Number Additional Info Location Last
NTIS  PB90-215948 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 15p
High resolution chromosome preparations from L5178Y TK+/- 3.7.2C mouse lymphoma cells were obtained by the use of acridine orange in the cell harvest procedure. With this technique it is possible to visualize over 500 bands in elongated mouse lymphoma chromosomes as compared to the approximately 230 bands visualized in metaphase preparations. High resolution mouse lymphoma chromosomes are described and chromosome rearrangements carried in the cell line are characterized by ideograms, which represent the position, number, size, and relative staining intensity of the G-band patterns. Use of elongated chromosomes of mouse lymphoma TK+/- mutants should facilitate analysis of cytogenetic effects associated with TK+/- -> TK-/- mutagenesis. (Copyright (c) 1989 Elsevier Science Publishers B.V. (Biomedical Division).)