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RECORD NUMBER: 2 OF 18

Main Title Bioassay-Directed Fractionation of Organic Contaminants in an Estuarine Sediment Using the New Mutagenic Bioassay, Mutatox (Trade Name).
Author Ho, K. T. Y. ; Quinn, J. G. ;
CORP Author Environmental Research Lab., Narragansett, RI. ;Rhode Island Univ., Kingston. Graduate School of Oceanography.
Publisher c1993
Year Published 1993
Report Number EPA/600/J-93/183 ;ERLN-1438;
Stock Number PB93-194504
Additional Subjects Bioassay ; Fractionation ; Organic compounds ; Sediments ; Water pollution detection ; Mutagens ; Toxicity ; Connecticut ; Chromatographic analysis ; Mass spectroscopy ; Chemical analysis ; Reprints ; Black Rock Harbor(Connecticut)
Holdings
Library Call Number Additional Info Location Last
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Status
NTIS  PB93-194504 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 10p
Abstract
Bioassay-directed fractionation of organic compounds was performed on an organic solvent extract of a contaminated estuarine sediment from Black Rock Harbor, Connecticut, using the new mutagenic bioassay, Mutatox. Chemical fractionation methods of the sediment extract included silica-gel-column chromatography (SGCC), followed by C-18 reverse phase HPLC. Compound identification was performed using GC-MS. Mutatox analyses indicated that four of the eight HPLC fractions contained mutagenic or epigenetic compounds. GC-MS analyses detected known mutagenic polycyclic aromatic hydrocarbons (PAHs) in two of the four mutagenic fractions. GC-MS analyses of the other two mutagenic fractions (one operational blank and one sediment fraction) showed no known mutagenic compounds. SGCC and HPLC, combined with the Mutatox bioassay, were useful in reducing both the number and the complexity of fractions analyzed by GC-MS. (Copyright (c) 1993 SETAC.)