Record Display for the EPA National Library Catalog

RECORD NUMBER: 3 OF 12

Main Title Effects of Nitrogen Source on Crude Oil Biodegradation.
Author Wrenn, B. A. ; Haines, J. R. ; Venosa, A. D. ; Kadkhodayan, M. ; Suidan, M. T. ;
CORP Author Cincinnati Univ., OH. Dept. of Civil and Environmental Engineering.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Publisher c1994
Year Published 1994
Report Number EPA-R-821029; EPA/600/J-94/489;
Stock Number PB95-136925
Additional Subjects Crude oil ; Biodegradation ; Nitrification ; Oil pollution ; Cultures(Biology) ; pH ; Ammonia ; Ammonium halides ; Nitric acid ; Reprints ; Respirometry
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Status
NTIS  PB95-136925 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
Abstract
The effects of NH4Cl and KNO3 on biodegradation of light Arabian crude oil by an oil-degrading enrichment culture were studied in respirometers. In poorly buffered sea salts medium, the pH decreased dramatically in cultures that contained NH4Cl, but not in those supplied with KNO3. The ammonia-associated pH decline was severe enough to competely stop oil biodegradation as measured by oxygen uptake. Regular adjustment of the culture pH allowed oil biodegradation to proceed normally. A small amount of nitrate accumulated in all cultures that contained ammonia, but nitrification accounted for less than 5% of the acid that was observed.