Record Display for the EPA National Library Catalog


Main Title Immunoblot Analysis of Exospore Polypeptides from Some Entomophilic Microsporidia.
Author Irby, W. S. ; Huang, Y. S. ; Kawanishi, C. Y. ; Brooks, W. M. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;North Carolina State Univ. at Raleigh.
Year Published 1986
Report Number EPA/600/J-86/052;
Stock Number PB86-201506
Additional Subjects Exposures ; Polypeptides ; Immunology ; Reprints ; Microsporidia ; Immunoblot analysis
Library Call Number Additional Info Location Last
NTIS  PB86-201506 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 10p
Immunological relationships between genera and species of microsporidia were examined by immunoblot analysis. Exospore polypeptides from 2 Nosema spp., 3 Vairimorpha spp., and 2 undescribed Vairimorpha-like spp. were analyzed. Gel electrophoresis and immunoblot analysis revealed that a variety of polypeptides, most between 15,000 and 90,000 molecular weight, were present on the exospore. The 3 Vairimorpha spp. were highly related immunologically to each other, but less so to the 2 Vairimorphalike undescribed spp. The 2 Nosema spp. immunologically distant from each other and from the Vairimorpha spp. Indirect evidence, however, indicated that many internal spore polypeptides present in both genera are similar. Cross-reactivity between exospore polypeptides from entomophilic microsporidia and antisera to a mammalian microsporidium, Encephalitozoon cuniculi was very limited.