Record Display for the EPA National Library Catalog


Main Title Maintenance and Stability of Introduced Genotypes in Groundwater Aquifer Material.
Author Jain, R. K. ; Sayler, G. S. ; Wilson, J. T. ; Houston, L. ; Pacia, D. ;
CORP Author Tennessee Univ., Knoxville.;Robert S. Kerr Environmental Research Lab., Ada, OK.
Year Published 1987
Report Number EPA-R-810485; EPA/600/J-87/136;
Stock Number PB88-148192
Additional Subjects Groundwater ; Biodeterioration ; Aquifers ; Microbiology ; Pseudomonas ; Contaminants ; Maintenance ; Stability ; Organic wastes ; Reprints ; Genotype ; Plasmids
Library Call Number Additional Info Location Last
NTIS  PB88-148192 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
Three indigenous groundwater bacterial strains and Pseudomonas putida harboring plasmids TOL (pWWO) and RK2 were introduced into experimentally contaminated groundwater aquifer microcosms. Maintenance of the introduced genotypes was measured over time by colony hybridization with gene probes of various specificity. On the basis of the results of colony hybridization quantitation of the introduced organisms and genes, all introduced genotypes were stably maintained at approximately 100,000 positive hybrid colonies g-1 of aquifer microcosm material throughout an 8-week incubation period. Concomitant removal of the environmental contaminants, viz., toluene, chlorobenzene, and styrene, in both natural (uninoculated) and inoculated aquifer microcosms was also demonstrated. The results indicate that introduced catabolic plasmids, as well as indigenous organisms, can be stably maintained in groundwater aquifer material without specific selective pressure for the introduced genotypes. These results have positive implications for in situ treatment and biodegradation in contaminated aerobic groundwater aquifers. (Copyright (c) Applied and Environmental Microbiology, 1987.)