Record Display for the EPA National Library Catalog


Main Title Ozone Effects on Alpha-1-Proteinase Inhibitor In vivo: Blood Plasma Inhibitory Activity is Unchanged.
Author Johnson, D. A. ; Winters, R. S. ; Woolley, T. ; Graham, D. ; Henderson, F. W. ;
CORP Author East Tennessee State Univ., Johnson City. ;North Carolina Univ. at Chapel Hill. School of Medicine.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1986
Year Published 1986
Report Number EPA/600/J-86/492;
Stock Number PB89-104855
Additional Subjects Ozone ; Alpha-1-antitrypsin ; Toxicity ; Enzyme inhibitors ; Elastase ; Exposure ; Respiration ; Blood plasma ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB89-104855 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 10p
The possible oxidative inactivation of human blood plasma alpha-1-proteinase inhibitor (PI) by inhaled ozone was assessed. Eleven male volunteers (non-smokers) were exposed to 0.5 ppm ozone for four hours on two consecutive days and ten control subjects were exposed to air under the same conditions. Blood plasma samples, drawn before and after treatment, were assayed for total PI, total protein and elastase inhibitory activity. The amount of active PI in plasma samples was quantitated by titrating porcine pancreatic elastase with increasing amounts of plasma. The importance of constructing titration curves for determinations of the type was demonstrated. No differences were noted in the ratios of total PI/ total protein or elastase inhibitory activity/total PI in the plasma from ozone exposed individuals relative to controls. Although inhaled ozone did not result in any significant decrease in the activity of blood plasma PI, these findings do not preclude the possibility of oxidative inactivation of PI in the lung. (Copyright (c) 1986 by Hemisphere Publishing Corporation.)