Record Display for the EPA National Library Catalog


Main Title Differential Mutant Quantitation at the Mouse Lymphoma TK and CHO (Chinese Hamster Ovary) 'HGPRT' Loci.
Author Moore, M. M. ; Harrington-Brock, K. ; Doerr, C. L. ; Dearfield, K. L. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
Publisher c1989
Year Published 1989
Report Number EPA/600/J-89/207;
Stock Number PB90-146242
Additional Subjects Mutations ; Toxicology ; Lymphomas ; Mice ; Graphs(Charts) ; Tables(Data) ; Hamsters ; Acrylates ; Reprints ; Hypoaxanthine phosphoribosyltransferase ; Thymidine kinase ; Toxic substances ; Dose-response relationships ; Chromosome aberrations
Library Call Number Additional Info Location Last
NTIS  PB90-146242 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 13p
Recent reports by several laboratories indicate that not all nonessential target loci are equally capable of detecting chromosomal mutations. The present study was undertaken to compare the ability of the tk locus in mouse lymphoma cells and the hgprt locus in CHO cells to quantitate chromosomal mutations. Seven known mutagens for the tk locus were selected for evaluation. From these investigations, it appears that only those compounds that induce significant numbers of large-colony tk mutants also induce significant numbers of hgprt mutants. Aberration analysis revealed that both the mouse lymphoma and CHO cells responded to the clastogenicity of the compounds and that neither cell line was clearly more sensitive to the clastogens tested. The present study adds to the increasing number of studies that support the view that the hemizygous nature of the hgprt locus permits the recovery of mutations primarily affecting the function of a single gene; whereas the heterozygous nature of the tk locus permits the recovery of both single gene and chromosomal mutations.