||Identification of Immunogens of 'Mycoplasma pneumoniae' by Protein Blotting.
Hu, Ping C. ;
Huang, Yuan-shen ;
Graham, Judith A. ;
Gardner, Donald E. ;
||Health Effects Research Lab., Research Triangle Park, NC.
Mycoplasma pneumoniae ;
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Proteins of Mycoplasma pneumoniae were separated by SDS-polyacrylamide gel electrophoresis and transferred to a nitrocellulose sheet by blotting. Sera obtained from infected hamsters and immunized rabbits were then incubated with the nitrocellulose strips. Proteins which are capable of eliciting antibodies were detected by indirect immunoradioautography using 125I-labeled antisera against hamster or rabbit IgG. Antibodies to seven immunogens were demonstrated in the sear of hamsters infected with M. pneumoniae by inhalation, while many more proteins were found to be capable of stimulating antibodies in rabbits immunized parenterally with mycoplasmas.