Record Display for the EPA National Library Catalog


Main Title Determination and Enhancement of Anaerobic Dehalogenation: Degradation of Chlorinated Organics in Aqueous Systems.
Author Palmer, D. T. ; Linkfield, T. G. ; Robinson, J. B. ; Sharak Genthner, B. R. ; Pierce, G. E. ;
CORP Author Battelle Columbus Div., OH.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Publisher Sep 88
Year Published 1988
Report Number EPA/600/2-88/054;
Stock Number PB89-110282
Additional Subjects Water pollution control ; Chlorine organic compounds ; Anaerobic bacteria ; Water treatment ; Performance evaluation ; Chemical analysis ; Bioengineering ; Genetics ;
Library Call Number Additional Info Location Last
NTIS  PB89-110282 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 59p
The report summarizes initial efforts: to isolate microorganisms capable of anaerobic dehalogenation; to examine the nutritional requirements of dehalogenating enrichments and a dehalogenating consortium; and to study the genetics of dehalogenation. Enrichments using secondary anaerobic digestor sludge containing either 3- or 4-chlorobenzoate were established with a variety of terminal electron acceptors. Degradation of 4-chlorobenzoate was not observed. Degradation of 3-chlorobenzoate (3CB) was observed after 10-23 weeks. Use of a 3CB degrading consortium revealed that the microorganisms capable of benzoate degradation and methanogenesis were lost but dechlorination continued. Genetic studies using a pure culture of strain DCB-1 found the strain to be suitable for cloning manipulations.