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RECORD NUMBER: 6 OF 16

OLS Field Name OLS Field Data
Main Title Differences in Detection of DNA Adducts in the 32P-Postlabelling Assay After Either 1-Butanol Extraction or Nuclease Pl Treatment.
Author Gallagher, J. E. ; Jackson, M. A. ; George, M. H. ; Lewtas, J. L. ; Robertson., I. G. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
Publisher c1989
Year Published 1989
Report Number EPA/600/J-89/471;
Stock Number PB91-115972
Additional Subjects Butanols ; Mutagens ; Chemical analysis ; Nitrogen organic compounds ; Aromatic hydrocarbons ; Solvent extraction ; Sensitivity ; Reprints ; Mutagenicity tests ; DNA damage ; Deoxyribonucleases ; Phosphorus 32 ; Benzo(a)pyrene ; Autoradiography
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB91-115972 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 03/04/1991
Collation 8p
Abstract
The use of nuclease P1 treatment and 1-butanol extraction to increase the sensitivity of the 32P-postlabelling assay for DNA adducts have been compared. Although similar results were obtained with the two methods for standard adducts formed with benzo(a)pyrene diol epoxide I, nuclease P1 treatment resulted in a significant reduction in detection of major adducts 1-amino-6-nitropyrene, 1-amino-8-nitropyrene, 2-aminofluorene, 2-naphthylamine and 4-aminobiphenyl modified DNAs, but not following the 32P-postlabelling analysis of 2-acetylaminofluorene modified DNA. These results suggest that at least initially, both modications of the 32P-postlabelling assay should be used for the detection of unknown adducts or for adducts derived from nitro-aromatics and aromatic amines. (Copyright (c) 1989 Elsevier Science Publishers Ireland Ltd.)