Record Display for the EPA National Library Catalog

RECORD NUMBER: 3 OF 25

Main Title Automated Analysis of Glutathione Peroxidase, S-Transferase, and Reductase Activity in Animal Tissue.
Author Jaskot, R. H. ; Charlet, E. G. ; Grose, E. C. ; Grady, M. A. ;
CORP Author Northrop Services, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1983
Report Number EPA-68-02-2566; EPA/600/J-83/346;
Stock Number PB86-217478
Additional Subjects Tissues(Biology) ; Laboratory animals ; Reprints ; Glutathione peroxidase ; S-trans-ferase ; Reductase ; Centrifugal analzer ; Spectrophotometer
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Status
NTIS  PB86-217478 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 5p
Abstract
A centrifugal analyzer and a spectrophotometer were compared for routine analysis of xenobiotic metabolizing enzymes glutathione (GSH) peroxidase, GSH-S-transferase, and GSH reductase. Lung, liver, and kidney from 60-day-old male rats were used as the source of enzymes. Linear regression analysis was used to assess the accuracy and precision of the centrifugal analyzer method in measuring enzyme activities. Biologically and statistically, the centrifugal analyzer proved to be acceptable for routine measurement of these GSH-dependent enzymes.