||Analysis of a Marine Fish Cell Line from a Male Sheepshead.
Gregory, Paula E. ;
Howard-Peebles, Patricia N. ;
Ellender, Rudolph D. ;
Martin, Billy J. ;
||University of Southern Mississippi, Hattiesburg. Dept. of Biology.;Environmental Research Lab., Gulf Breeze, FL.
||EPA-R-804527; EPA-600/J-80-130 ; ERL.GB-0089
Cell lines ;
Archosargus probatocephalus ;
||Some EPA libraries have a fiche copy filed under the call number shown.
Chromosomes from consecutive culture passages of a developing cell line from fin fibroblasts of a male sheepshead (Archosargus probatocephalus) were analyzed. It was demonstrated that the modal chromosome number is 48. The chromosome types found in these cells included 8 submetacentrics, 8-12 acrocentrics, and 28-32 telocentrics. Evidence suggests that the primary type of chromosomal alteration that occurred in cultured SHR-1 cells is aneuploidy; a large metacentric chromosome of undetermined origin was observed.