Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Identification of Immunogens of 'Mycoplasma pneumoniae' by Protein Blotting.
Author Hu, Ping C. ; Huang, Yuan-shen ; Graham, Judith A. ; Gardner, Donald E. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1981
Report Number EPA-600/J-82-029;
Stock Number PB83-125062
Additional Subjects Bacteria ; Protein ; Immunology ; Identifying ; Electrophoresis ; Hamsters ; Rabbits ; Antibodies ; Reprints ; Mycoplasma pneumoniae ; Blotting ; Immunogens
Library Call Number Additional Info Location Last
NTIS  PB83-125062 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/23/1988
Collation 10p
Proteins of Mycoplasma pneumoniae were separated by SDS-polyacrylamide gel electrophoresis and transferred to a nitrocellulose sheet by blotting. Sera obtained from infected hamsters and immunized rabbits were then incubated with the nitrocellulose strips. Proteins which are capable of eliciting antibodies were detected by indirect immunoradioautography using 125I-labeled antisera against hamster or rabbit IgG. Antibodies to seven immunogens were demonstrated in the sear of hamsters infected with M. pneumoniae by inhalation, while many more proteins were found to be capable of stimulating antibodies in rabbits immunized parenterally with mycoplasmas.